Abstract: Coal mine methane tends to accumulate, leading to gas explosions and other disasters and posing a serious threat to the safety of mine personnel. To address this problem, two methane-oxidizing bacteria (MOB) are isolated from the coal mine environment through enrichment culture with methane as the sole carbon source,and are identified as Methylobacter tundripaludum SV96^T (strain T) and Methylophilus sp. D22 (strain S). The culture conditions of the two strains are optimized by the one-factor-at-a-time method, and their environmental adaptability and reaction kinetics were investigated. The results showed that both strains canutilize peptone and methanol for growth, and these substances can serve as auxiliary carbon sources for scale-up cultivation. Meanwhile, the two strains differ in environmental adaptability: strain S exhibits strong environmental adaptability under various experimental conditions, whereas Strain T is relatively sensitive to environmental changes. The optimal culture temperature for both strains is 35 ℃. For strain T, the optimal pH and inoculation amount are 7 and 10%, respectively, and 8 and 5% for strain S. Under the optimal culture conditions, the methane consumption rates of strains T and S reach （88.83±4.8）% and （83.21±5.1）%, respectively. Kinetic analysis shows that the overall growth of strains T and S conformed to the Logistic growth model, with maximum specific growth rates b_max in the logarithmic phase of 0.029 9 h⁻¹ and 0.035 3 h⁻¹, respectively. A linear positive correlation is observed between growth and methane consumption, and optimization suggestions and application strategies proposed from the perspective of mass transfer. The CH₄ oxidation process exhibits a conversion characteristic in which the molar consumption rate of CH₄ is approximately twice the molar production rate of CO_2, which is consistent with the metabolic pattern of methanotrophs partitioning carbon between oxidative energy production and growth. The above results provide an optimization scheme for methane emission reduction projects, including coal mine methane control. In the experiment simulating the goaf environment, both strainsT and S reduce the CH₄ volume fraction to below 0.1% within 66 h, and fitting reveals that their metabolic processes follow the Logistic decay kinetics. The experiment shows that strain S exhibitsstronger environmental adaptability and a stronger rapid response capability, whereas strain T requires prior colonization and survival under stable operating conditions before achieving efficient metabolism. The results provide strain resources and a theoretical basis for the microbial treatment technology of CH₄ in goafs.