Abstract: In order to distinguish a hydrochemistry feature difference between the each aquifer in a mine, a study was conducted on the 3D fluorescence spectrum f eatures of the solubility organic matter in the underground water. The results showed that in the surface ground water and the Quaternary water, there were fluorescenc e peaks of each type organic matter and the fluorescence intensity was high.lIn the sandstone water, there was no tyrosine fluorescence peak, the fluorescence intensit y of the hydrophobic organic acid and the marine humic acid was 99.75 QSU and 56.50 QSU individually, which were lower than the overburden water value.In the Ordo vician limestone water, TOC and UV254 were obviously lower than other aquifer and there was no fluorescence peak of tyrosine, hydrophobic organic acid and marine h umic acid.The tryptophan fluorescence intensity was higher than in other aquifer and the value was 175.23 QSU.With the 3D fluorescence spectrum features, the hydro chemistry features of Ordovician limestone water and each aquifer in above strata could be obviously distinguished and the water inrush sources could be well and acc urately identified.