Abstract: In order to understand the formation mechanism of organic carbon in reclaimed soil,the ecological restoration application status and carbon sequestration mechanism of arbuscular mycorrhizal fungi in soil were analyzed.The key factors affecting organic carbon sequestration in reclaimed soils of the mining areas and related research progress are reviewed from the aspects of vegetation roots,enzyme activities and soil aggregates.Among them,the glomalin-related soil protein produced by arbuscular mycorrhizal fungi improves the accumulation of organic carbon in the soil by promoting the formation of stable aggregates of soil particles. The plant roots accelerate the conversion rate of organic matter by the catalytic action of invertase,urease and others,with the participation of a large number of underground organisms.However,the current research on soil organic carbon mostly focuses on the dynamic change of organic carbon and the analysis of related impact factors.In the future,the carbon balance of reclaimed soil can be further explored from the mineralization and degradation mechanism of organic carbon.